The Nano Eye Device (NED) has been designed to speed up and simplify “Digital Transformation” in healthcare applications, paying particular attention to real time remote digital imaging-based diagnostics, either for Molecular Diagnostics or for Digital Pathology application. NED is a unique, integrated, open, real-time and fully digital microscopy IoT platform also detecting sub-diffractive nanoparticles designed by the user experience. NED’s open hardware and software architecture also allows to third parties developer to build medical diagnostic applications and to quick prototyping and development of Artificial Intelligence driven diagnostic applications.